MENÚ
AR | ARS
AR | ARS
Está a punto de abandonar este sitio.
Por favor, tenga en cuenta que su carro de la compra actual no ha sido guardado todavía y no podrá ser restablecido en el nuevo sitio ni cuando regrese. Si desea guardar su carro de la compra, inicie sesión en su cuenta.
myEppendorf
No se han encontrado resultados
Sugerencias de búsqueda
![](/media/MAIN/01-Lab-Academy/08_NW_GettyImages-544457019_RGB_Filter_700.jpg)
Cell Culture FAQ: How does DMSO affect your cells?
Academia de laboratorio
- Biología celular
- Cultivo celular
- Incubadores de CO2
- Consumibles para cultivo celular
- Preguntas más frecuentes
This article was published first in "Inside Cell Culture" , the monthly newsletter for cell culture professionals. Find more interesting articles about CO2 incubators on our page "FAQs and material on CO2 incubators" .
Answer by Dr. Jessica Wagener, Application Specialist Cell Handling at Eppendorf
DMSO (Dimethyl Sulfoxide) is a polar, aprotic organic solvent that is commonly used as a cryoprotectant because of its membrane penetrating and water displacement properties. It is added to cell culture media to reduce ice formation and thereby prevent cell death during the freezing process.
Empiric studies report that during an initial freezing phase a temperature reduction of about 1°C/min is optimal. In vitro, DMSO at concentrations higher than 10% (v/v) is reported to induce membrane pore formation and apoptosis through caspase-9 and -3 activation. The response will depend on the type of cell or cell line.
Time is thus a critical factor when you freeze and thaw cells, because you want to minimize their exposure to DMSO. A trypan blue test can help to detect the cells' viability after thawing.
Hematopoietic cells, embryonic stem cells and many hybridoma cell lines seem to be particularly vulnerable to DMSO, as it is also a differentiation inductor.
Answer by Dr. Jessica Wagener, Application Specialist Cell Handling at Eppendorf
DMSO (Dimethyl Sulfoxide) is a polar, aprotic organic solvent that is commonly used as a cryoprotectant because of its membrane penetrating and water displacement properties. It is added to cell culture media to reduce ice formation and thereby prevent cell death during the freezing process.
Empiric studies report that during an initial freezing phase a temperature reduction of about 1°C/min is optimal. In vitro, DMSO at concentrations higher than 10% (v/v) is reported to induce membrane pore formation and apoptosis through caspase-9 and -3 activation. The response will depend on the type of cell or cell line.
Time is thus a critical factor when you freeze and thaw cells, because you want to minimize their exposure to DMSO. A trypan blue test can help to detect the cells' viability after thawing.
Hematopoietic cells, embryonic stem cells and many hybridoma cell lines seem to be particularly vulnerable to DMSO, as it is also a differentiation inductor.
Leer más
Leer menos